RNA干扰

RNAi(RNA interference)即RNA干扰是指一种分子生物学上由双链RNA诱发的基因沉默现象,其机制是通过阻碍特定基因的转译或转录来抑制基因表达。具体指的是通过反义RNA 与正链RNA形成双链RNA特异 性地抑制靶基因的转录后表达的现象,它通过人为地引入与内源靶基因具有相同序列的 双链RNA(有义RNA 和反义RNA),从而诱导内源靶基因的mRNA降解,达到阻止基因表达的目的。

慢病毒递送设计的shRNA和在哺乳动物细胞中的RNA干扰的机制
枢密科技可提供多种shRNA表达载体,如腺相关病毒载体、慢病毒载体、腺病毒载体以及普通的真核表达载体等。

参考文献:

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